Frog Ringer's Solution

12 October 2003

Chemical

Molec. Wt.

Concentration

to make 500 ml

to make 1 liter

to make 2 liters

HEPES (a buffer)

238

5mM

0.595 g

1.19 g

2.38 g

NaCl

58

125mM

3.63 g

7.25 g

14.5 g

KCl

75

3mM

0.113 g

0.225 g

0.450 g

CaCl2

147

1.8mM

0.113 g

0.265 g

0.53 g

Glucose (Dextrose)

180

10mM

0.90 g

1.80 g

3.60 g

MgCl2

203

1.6mM

0.162 g

0.324 g

0.648 g

Procedure:

Use only glassware that is labeled "Ringer's Only."

Rinse weighing spatula with distilled water and dry with a Chem-Wipe before and after scooping each chemical.

Set up a beaker with stir bar on a stir plate. Add about 90% of the final volume of distilled water to the beaker and have the water stirring while adding the ingredients.

Once all chemicals have been added, bring to the final volume in a graduated cylinder.

Return the Ringer's solution the beaker on the stir plate and adjust the pH while agitating.

Rinse of the pH probe with distilled water, blot dry with a Chem-wipe and suspend in the Ringer's Solution.

pH should be adjusted to 7.3 to 7.45 for amphibians with 1M NaOH.

Rinse pH probe with distilled water before returning to storage solution.

Transfer Ringer's solution to a bottle with cap, label, and store in refrigerator.